This deliverable provides an overview of liverworts selected and collected for downstream analyses within the BRYOMOLECULES project. Material was primarily obtained in Sweden, with additional collections from Iceland and Italy, accompanied by details such as sampling locations, coordinates, dates, and collector information.

Picture: Metzgeria furcata taken by Nils Cronberg

Establishment of Sterile Accessions

Sterile accessions were generated by surface sterilising sporophytes or vegetative plant material using a 0.05% Sodium Dichloroisocyanurate solution for 1–2 minutes, followed by rinsing with sterile deionised water. Cultures are maintained on 1% agar in a modified Rudolph et al. (1988) medium containing Benomyl to limit fungal contamination. Erythromycin was applied when required to remove cyanobacterial infections. All accessions are kept under controlled LED-tube lighting, 16/8 day/night cycles, and a mean temperature of 20°C.

Overview of Taxonomic Diversity

The collection comprises a diverse array of thallous and leafy liverworts, including their respective taxon names. Synonyms used during collection are included.